Prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency in neonates in Bunda Women's and Children's Hospital, Jakarta, Indonesia

  • Risma Kerina Kaban Department of Child Health, University of Indonesia Medical School/Dr. Cipto Mangunkusumo Hospital, Jakarta
  • Veronica Wijaya Department of Child Health, University of Indonesia Medical School/Dr. Cipto Mangunkusumo Hospital, Jakarta
Keywords: G6PD, screening, prevalence, neonates, Jakarta, Indonesia


Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most connnon enzyme deficiency in the world. Itis a risk factor for hyperbilirubinemia in neonates, which can cause serious complications such as bilirubinô€—induced encephalopathy or kernicterus. WHO recommends universal neonatal screening for G6PD deficiency when the frequency exceeds 3ô€—5% of male newborns.

Objective To assess the prevalence of G6PD deficiency among neonates in Bunda Women and C hildren Hospital (Bunda WCH), Jakarta, in order to detennine if there is a need for routine G6PD neonatal screening.

Methods This is a cross-sectional and retrospective study; infants' data were obtained from medical records. From January 2009 to May 2010, all neonates in Bunda WCH were screened for G6PD deficiency on the yd day of life. Blood samples were collected using filter papers. We considered a result to be nonnal if it exceeded 3.6 U/g Hb.

Results A total 1802 neonates were screened. We found 94 neonates (5.2%) with G6PD deficiency. Out of 943 males, 59 (6.26%) were G6PD deficient, and out of 859 females, 35 (4.07%) were G6PD deficient. We observed that prevalence of G6PD deficiency according to sex distribution was significantly higher in males than females (6.26% vs. 4.07%, P=0.037). There was no significant difference in the risk for severe hyperbilirubinemia between the G6PD deficient infants and the nonnal infants (P=0.804).

Conclusions The frequencies of G6PD deficiency were 6.26% of male neonates and 4.07% of female neonates. We recommend universal neonatal screening for G6PD deficiencies in Jakarta since our findings exceed the WHO recommendation for routine testing.


1. Luzzato L, Mehta A. Glucose-6-phosphate dehydrogenase deficiency. In: Scriver CR, Beaudet AL, Sly W S, Valle D, editors. T he metabolic and molecular basis of inherited disease. New York: McGraw.Hill; 1995. p.3367-98.
2. Nkhoma ET, Poole C, Vannappagari V, Hall SA, Beutler E. The global prevalence of glucose 6􀁩phosphate dehydrogenase deficiency: a systematic review and metaanalysis. Blood Cells Mol Dis. 2009;42:267-78.
3. Mohammadzadeh A, Jafarzadeh M, Farhat SA, Keramati MR, Badiee Z, Esmaily HO, et al. Prevalence of glucose 6-phosphate dehydrogenase deficiency in neonates of Northeast Iran. J Chinese Clin Med. 2009;4:448-51.
4. Luzzato L. Glucose 6-phospate dehydrogenase deficiency: from genotype to phenotype. Hematologica. 2006;91:1303-6.
5. Minareci E, Uzunoglu S, Minareci o. Incidence of severe glucose 6-phosphate dehydrogenase (G6PD) deficiency in countryside villages of T he Central City of Manisa, Turkey. Eur J Gen Med. 2006;3:5-10.
6. Castro S, Weber R, Dadalt V, Tavares V, Giugliani R. Prevalence of G6PD deficiency in newborns in the south of Brazil. J Med Screen. 2006;13:85-6.
7. Soemantri AG, Saha S, Saha N, Tay JS. Molecular variants of red cell glucose-6-phosphate dehydrogenase deficiency in Central Java, Indonesia. Hum Hered. 1995;45: 346-50.
8. Matsuoka H, Arai M, Yoshida S, Tantular IS, Pusarawati S, Kerong H, et al. Five different glucose-6-phosphate dehydrogenase (G6PD) variants found among 11 g6pd􀁩 deficient persons in Flores Island, Indonesia. J Hum Genet. 2003;48:541-4.
9. Kawamoto F, Matsuoka H, Kanbe T, Tantular IS, Pusarawati S, Kerong HI, et al. Further investigation of glucose-6-phosphate dehydrogenase variants in Flores Island, Eastern Indonesia. J Hum Genet. 2006;51:952-7.
10. Shimizu H, Tamam M, Soemantri A, Ishida T. Glucose-6-phosphate deficiency and Southeast Asian ovalocytosis in asymptomatic Plasmodium carriers in Sumba Island, Indonesia. J Hum Genet. 2005;50:420-4.
11. Segel GB. Enzymatic defects Glucose-6-phospate dehydrogenase (G6PD) and related deficiencies. In: Behnnan RE, Kliegman RM, Jenson HB, editors. Nelson textbook of pediatrics. 17th Ed. Philadelphia: Saunders, 2004; p. 1636-8.
12. Kaplan M, Beutler E, Vreman HJ, Hammennan C, Levy-Lahad E, Renbaum P, et al. Neonatal hyperbilirubinemia in glucose-6-phosphate dehydrogenase􀁩deficient heterozygotes. Pediatrics. 1999;104:68-74.
13. Kaplan M, Hanunennan C. Evaluation of glucose 6-phosphate dehydrogenase deficiency a hidden risk for kernicterus. Semin Perinatol. Ann Trop Pediatr. 2004;28:356-64.
14. Johnson L, Bhutani VK, Karp K, Sivieri EM, Shapiro SM. Clinical report from the pilot USA kernicterus registry (1992 to 2004). J Perinatol 2009;29(Suppl):S25-S45.
15. Slusher TM, V reman HJ, McLaren DW, Lev.ison LJ, Brown AK, Stevenson DK. Glucose-6-phosphate dehydrogenase deficiency and carooxyhemoglobin concentrations associated v.ith bilirubin related morbidity and death in Nigerian infants. J Pediatr. 1995;126:102-8.
16. Raho JA. T he changing moral focus of newborn screening: An ethical analysis by the president's council on bioethics. Appendix, newborn screening: An international survey.c201O. Available from: http://bioethics.georgeto\ screening/the changing moral focus
of newborn screening􀁩 appendix - joseph raho.pdf.
17. WHO Working Group. Glucose􀁩6􀁩Phosphate Dehydrogenase Deficiency. Bull World. Health Organ. 1989;67:601-11.
18. Kit-Insert Bio-Rad Microplate Neonatal G6PD Screening Assay, Bio-Rad Laboratories.
19. Bhutani VK, Johnson LH, Sivieri EM. Predictive ability of a predischarge hour-specific serum bilirubin for subsequent significant hyperbilirubinemia in healthy tenn and near􀁩tenn newborns. Pediatrics. 1999;103:6-14.
20. Kaplan M, Leiter C, Hammerman C, Rudensky B. Comparison of commercial screening tests for glucose􀁩6􀁩 phosphate dehydrogenase deficiency in the neonatal period. Clin Chern. 1997;43:1236-7.
21. Reclos GJ, Hatzidakis CJ, Schulpis KH. Glucose 6-phosphate dehydrogenase deficiency neonatal screening: preliminary evidence that a high percentage of partially deficient female neonates are missed during routine screening. J Med Screen. 2000;7:46-51.
22. Nock ML, Johnson EM, Krugman RR, Di Fiore JM, Fitzgerald S, Sandhaus LM, et al. Inplementation and analysis of a pilot inô€€»hospital newborn screening program for glucoseô€€»6ô€€»phosphate dehydrogenase deficiency in the United States. J Perinatol. 2010;1-6.
How to Cite
Kaban R, Wijaya V. Prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency in neonates in Bunda Women’s and Children’s Hospital, Jakarta, Indonesia. PI [Internet]. 28Feb.2011 [cited 12Aug.2022];51(1):29-3. Available from:
Received 2016-10-17
Accepted 2016-10-17
Published 2011-02-28