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establish because of its similar symptoms to those of respiratory
infection caused by other viruses. The “gold standard” for the
diagnosis of influenza is viral culture, which takes time to gain
the result and is expensive as well. A simple, rapid, and easily
used tool for detection of influenza virus type A and B is needed.
Objective To assess the accuracy of lateral-flow immunoassay with
Quick Vue Influenza A+B ® in detecting influenza virus of type
A and B.
Methods This was an observational study designed for diagnostic
test. The subjects were children aged 0-14 years old presenting
with acute respiratory infection in primary Health Care Jetis ,
Godean I, Godean II and Prof. Dr. Sardjito Hospital Yogyakarta,
from October 2005 to May 2007. Specimens were collected from
both the anterior nares and the throat by physicians for lateral-
flow immunoassay with Quick Vue Influenza A+B ® and viral
culture as gold standard. Lateral-flow immunoassay was done in
each study centre, nasal specimen was placed in an extraction
reagent tube and sent to NAMRU II laboratory.
Results There were 255 children enrolled in this study. Lateral-
flow immunoassay by Quick Vue Influenza A+B ® has sensitivity
70% (CI95% 6;83%), specificity 93% (CI95% 90;97%), positive
predictive value 68% (CI95% 54;82%), negative predictive value
94% (CI95% 91;97%), positive likelihood ratio 10,56 (CI95%
6,14;18,19) and negative likelihood ratio 0,32 (CI95% 0,21; 0,51).
Conclusion Lateral-flow immunoassay (Quick Vue Influenza
A+B ® ), nasal swab specimen is not accurate to detect influenza
virus A and B in children.
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