Differences of nucleotide and amino acid sequences of nucleoprotein (N) gene between wild-type measles virus and vaccine virus in Indonesia

  • Made Setiawan
  • Agus Sjahrurachman
  • Fera Ibrahim
  • Agus Suwandono
Keywords: measles, wild-type, vaccine-type, n gene


Background Measles virus is a member of genus morbilivirus
which belongs to family paramyxovirus with negative, single-
strand RNA genome. RNA is packed by nucleocapsid (N) protein.
The N protein is very important for RNA replication and
translation. Abnormality in N protein will induce abnormality in
virus replication.
Objective This study aimed to explore the differences of
nucleotide sequence of N gene and amino acid sequences of N
protein between wild-type measles virus (G2, G3 and D9) and
vaccine virus (CAM-70, Schwarz and Edmonston-wt)
Methods The exctraction and amplification of the gene were
conducted in the laboratory using biomolecular technology. The
gene and protein analysis were conducted using the bioinformatic
Results The results showed that more differences were found
between nucleotide sequences of N gene of wild-type measles
virus against CAM-70 vaccine virus (77 – 79 nucleotides)
compared against Schwarz and Edmonston-wt vaccine virus (71-
74 nucleotides). Likewise, more differences were also observed
between amino acid sequences of N protein of wild-type measles
virus against CAM-70 vaccine virus (18-24 residues) compared
against Schwarz and Edmonston-wt vaccine virus (17-23 residues).

Author Biographies

Made Setiawan
Department of Child Health, Infectious Diseases Hospital Prof.
Sulianti Saroso, Jakarta, Indonesia
Agus Sjahrurachman
Department Microbiology, Medical School, University of Indonesia, Jakarta, Indonesia
Fera Ibrahim
Department Microbiology, Medical School, University of Indonesia, Jakarta, Indonesia
Agus Suwandono
National Institute Health Research and Development,


1. Tyrell DIJ, Norrby F. Structural polypeptides of measles virus.
J Gen Virol 1978;39:219-229.
2. Rima BK. The proteins of morbilli viruses. J Gen Virol
3. Bellini WJ, Rota JS, Rota PA. Virology of measles virus. J
Infct Dis 1994;170:S15-23.
4. Lamb RA, Kolakofsky D. Paramyxoviridae: the viruses and
their Replication; In Field Virology; Third ed. Vol.1;
Philadelphia-New York: Lippincott-Raven, 1996; p.1177-
5. Griffin, DE, Bellini, WJ. Measles Virus. In: Fields Virology.
3 rd ed. Philadelpia-New York: Lippincott-Raven, 1996; p.
6. Bankamp B, Horikami SM, Thompson PD, Huber M, Billeter
M, Moyer SA. Domains of the measles virus N protein
required for binding to P protein and self-asembly. Virology
7. Giraudon P, Jacquier MF, Wild TF. Antigenic analysis of
african measles virus field isolates: identification and
localization of one conserved and two variable epitope site
on the NP protein. Virus Res 1988;18:137-152.
8. Baczko K, Pardowitz I, Rima BK, Meulen VT. Constan and
variable regions of measles virus proteins encoded by the
nucleocapsid and phosphoprotein gen derived from lytic and
persistent viruses. Virology 1992;190:469-474.
9. Rota PP, Bloom AE, Vanehiere JA, Bellini WJ. Evolution of
the nucleoprotein and matrix gene of wild-type strains of
measles virus isolated from recent epidemics. Virology 1994;
10. Taylor MJ, Godfrey E, Baczko K, ter Meulen V, Wild TF,
Rima BK. Identification of several different lineages of
measles virus. J Gen Virol 1991;72:83-8.
11. WHO: Expanded program on immunization. Standardization
of the nomenclature for describing the genetic
characterization of wild-type measles viruses. Weekly
Epidemiological report 2001;76:241-248.
12. Rota JS, Heath JL, Rota AP, King GE, Celma ML, Carabana
J, et al. Molecular epidemiology of measles virus: Identification
of pathways of transmission and implications for measles
elimination. J Infec Dis 1996;173:32-7.
13. Grave M, Griffin DE, Johnson RT, Hirsch RL, Soriano L,
Roedenbeck S, et al. Development on antibody to measles
virus polypeptides during complicated and uncomplicated
measles virus infections. J Virol 1984;49:409-412.
14. Beauverger P, Chadwick J, Buckland, R, Wild TF. Serotype-
spesific and canine distemper virus cross-reactive H-2KK-
restricted cytotoxic T lymphocyte epitopes in the measles
virus nucleoprotein. Virology 1994;203:172-7.
15. Rota JS, Wang ZD, Rota PA, Bellini WJ. Comparison of
sequnces of the H, F, and N coding genes of measles virus
vaccin strains. Virus Res 1994;31:317-30.
16. Tamin A, Rota PA, Wang Z, Heath JL, Anderson LJ, Bellini
WJ. Antigenic analysis of current wild type and vaccine
strains of measles virus. J Infect Dis 1994;170:795-801.
17. Litbangkes DepKes RI.Laporan hasil genotipe virus campak
yang dikirim oleh WHO. (2002)
18. Setiawan M. Analisis genetik dan antigenik virus campak
liar dan virus vaksin di Indonesia [Dissertation]. Jakarta:
Fakultas Kedokteran Universitas Indonesia; 2005.
19. Komase K, Suzuki N, Nakayama T, Miki K, Kawanishi R,
and Fukuda K. Genom sequence of measles virus. NCBI no.
accession: AB046218 (2001).
20. Coligan JE, Kruisbeek AM, Margulies DH, Shevach, EM,
Strober W. Current protocols in immunology. Vol. I. National
Institut of Health; Published by Current Protocols Wiley, (1996).
21. Flint SJ, Enquist LW, Krug RM, Racaniello VR, Skalka AM.
Principle of virology, moleculer biology, pathogenesis and
control. Washington DC: ASM press; 2000. p. 479-515.
How to Cite
Setiawan M, Sjahrurachman A, Ibrahim F, Suwandono A. Differences of nucleotide and amino acid sequences of nucleoprotein (N) gene between wild-type measles virus and vaccine virus in Indonesia. PI [Internet]. 1May2008 [cited 12Aug.2022];48(2):81-. Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/483
Received 2016-09-07
Accepted 2016-09-07
Published 2008-05-01