UCP3 gene polymorphism and insulin resistance in obese female adolescents

  • Dian Eurike Department of Histology and Cell Biology, Gadjah Mada University Medical School, Yogyakarta
  • Harry Freitag Luglio Muhammad Department of Health Nutrition, Gadjah Mada University Medical School, Yogyakarta
  • Rina Susilowati Department of Histology and Cell Biology, Gadjah Mada University Medical School, Yogyakarta
  • Madarina Julia Department of Child Health, Gadjah Mada University Medical School, Yogyakarta
DOI: https://doi.org/10.14238/pi52.3.2012.152-6
Keywords: UCP3, polymorphism, insulin resistance, obesity, female adolescents

Abstract

Background A previous study on obese female adolescents in
Yogyakarta done in the year 2007 suggests that genetic factors
might influence insulin resistance. One genetic factor that has
been associated \\lith insulin resistance in other populations is
the ô€55C;T polymorphism in the uncoupling protein 3 (UCP3)
gene.
Objective To investigate an association between the ô€55C;T
polymorphism in the UCP3 gene and insulin resistance in obese
female adolescents in Yogyakarta.
Methods A total of79 obese female adolescents were enrolled in
this crossô€sectional study. Genotyping of the ô€ 5 SC/T polymorphism
in the UCP3 gene was performed by polymerase chain reactionô€
restriction fragment length polymorphism (PCRô€RFLP).
Results The TiT homozygous individuals had a higher risk of
insulin resistance (OR 2.3; 95% CI 0.4 to 14.1), as well as higher
fasting insulin concentration and homeostatic model assessment
for insulin resistance (HOMAô€IR) compared to individuals v.ith
other genotypes. The T allele carriers also had a higher risk of
insulin resistance (OR 1.3; 95% CI 0.7 to 2.5), as well as higher
fasting insulin concentration and HOMAô€ IR compared to C
allele carriers. However, none of these results were statistically
significant (P > 0.05).
Conclusion The TiT genotype and T allele of the UCP3 gene
ô€55CiT polymorphism was not significantly associated increased
risk of insulin resistance in obese female adolescents in Yogyakarta.
[Paediatr lndanes. 2012;52:152-6].

References

t. Daniels SR, Arnett DK, Eckel RH, Gidding SS, Hayman LL,
Kumanyika S, et al.O verweight in children and adolescents:
pathophysiology, consequences, prevention, and treatment.
Circulation. 2005;ll U999,2012.
2. Dietz WHo Overweight in childhood and adolescence. N
Engl J Med. 2005;350,855,7.
3. Formiguera X, Canton A. Obesity: epidemiology and clinical
aspects. Best Pract Res Clin GastToenterol. 2004;18:1125A5.
4. Eyzaguirre F, Mericq V. Insulin resistance markers in children.
Hottn Res. 2009;71,65,74.
5. Julia M, Huriyari E, Susilowari R.H ubungan antara resistensi
insulin dengan pola makan, pola akrivitas, pola menstruasi
dan tekanan darah remaja wan ita obes. Laporan Penelirian
Dana Masyarakat FK UGM 2008. Yogyakarta: Universitas
Gadjah Mada; 2008. p. 1,23.
6. Sulistyoningrum E. Hubungan sind rom metabolikpada remaja
putri obes dengan sindrom metabolik pada orang tua [Master's
thesis]. [Yogyakarta]: Universitas Gadjah Mada; 2009.
7. Ricquier D, Bouillaud E Mitochondrial uncoupling proteins:
from mitochondria to the regulation of energy balance. J
Physiol. 2000;529,3,10.
8. Femandez􀂣Veledo S, Nieto􀂣Vazquez C de Castro J, Ramos
MP, Bruderlein S, Moller P, et al. H yperinsulinemia induces
insulin resistance on glucose and lipid metabolism in a human
adipocyric cell line: paracrine interaction with myocytes. J
Clin Endocrinol Metab. 2008;93,2866,76.
9. Schrauwen P, Xia J, Walder K , Snitkers S, Ravussin E.
A novel polymorphism in the proximal UCP3 promoter
region: effect on skeletal muscle UCP3 mRNA expression
and obesity in male nondiabetic Pima Indians. Int JObes.
1999;23, 1242,5.
10. Gable DR, Stephens JW, Cooper JA, Miller OJ, Humphrie
SE. Variation in the UCP2􀁡UCP3 gene cluster predicts the
development of type 2 diabetes in healthy middle􀂣aged men.
Diabetes. 2006;55,1504, It.
11. Ochoa MC, Santos JL, Azcona C, Moreno􀂣Aliaga MJ,
Marrinez􀂣Gonzalez MA, Martinez JA, et al. Association
between obesity and insulin resistance with UCP2􀂣UCP3
gene variants in Spanish children and adolescents. Mol Genet
Metab.2007;920351,8.
12. Roman DAL, Aller R, Jauregui 01, Sagrado MG, Vicente
RC, de la Fuente B, et al. Relation of 􀂣55cr polymorphism of
uncoupling protein 3 gene with fat mass and insulin resistance
in morbidly obese patients. Metab Clin Experiment.
2010;59,608,12.
13. Meirhaeghe A, Amouyel P, Helbecque N, Cottel D, Otabe S,
Froguel P, eta!' An uncoupling protein 3 gene polymorphism
associated with a lower risk of developing type 2 diabetes
and with atherogenic lipid profile in a French cohort.
Diabetologia. 2000;43, 1424,8.
14. Keskin M, Kurtoglu S, Kendirci M, Atabek E, Yazici C.
Homeostasis model assessment is more reliable than the fasting
glucose/insulin ratio and quantitative insulin sensitivity check
index for assessing insulin resistance among obese children and
adolescents. Pediatrics. 2005; 115 :500􀁛 3.
15. Sambrook J, Fritsch RF, Maniatis T. Molecular cloning. A
laboratory manual. 2nd ed. New York: Cold Spring Harbor
Lab Press; 1989. p. 916-18.
16. Dalgaard LT, Sorensen TIA, Drivsholm T, oorch􀁛Johnsen K,
Andersen T, Hansen T, et al. A prevalent polymorphism in
the promoter of the UCP3 gene and its relationship to body
mass index and long term body weight change in the Danish
population. j Clin Endoctinol Metab. 2001;86,1398-1402.
17. Aller R, de Luis DA, Izaola I, Sagrado MG, Conde R, Alvarez
T, et al. Role of 􀁛55cr polymorphism ofUCP3 gene on non􀁛
alcoholic fatty liver disease and insulin resistance in patients
with obesity. Nutr Hosp. 2010;25,572-6.
18. Hamada T, Kotani K, Fujiwara S, Sano Y, Domichi M, Tsuzaki
K, et al. The cormnon 􀁛 55 CiT polymorphism in the promoter
region of the uncoupling protein 3 gene reduces prevalence
of obesity and elevates serum high􀁛density lipoprotein
cholesterol levels in the general Japanese population. Metab
Clin Experiment. 2008;570410-5.
19. Otabe S, Clements K, Dina C, Pelloux V, Guy􀁛Grand, B,
Froguel P, et al. A genetic variation in the 5' flanking region
of the UCP3 gene is associated \\lith body mass index in
humans in interaction \\lith physical activity. Diabetologia.
2000;43,245-9.
20. jun HS, Kim IK, Lee Hj, Kang Hj, Kim jR, Shin HD, et al.
Effects ofUCP2 and UCP3 variants on the manifestation of
overweight in Korean children. Obesity. 2008;7:355􀁛62.
21. Ban Hj, Heo jY, Oh KS, Park Kj. Identification of type 2
diabetes associated combination of SNPs using support vector
machine. BMC Genet. 2010;11:1􀁛11.
Published
2012-06-30
How to Cite
1.
Eurike D, Muhammad H, Susilowati R, Julia M. UCP3 gene polymorphism and insulin resistance in obese female adolescents. PI [Internet]. 30Jun.2012 [cited 23Dec.2024];52(3):152-. Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/382
Issue
Vol 52 No 3 (2012): May 2012
Section
Articles

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Received 2016-08-30
Accepted 2016-08-30
Published 2012-06-30