Antigenic differences between wildtype measles viruses and vaccine viruses in Indonesia
Keywords:
measles virus, antigenic, measles vaccine, neutralization, ELISA
Abstract
Background Measles virus has a single, negative strand RNAgenome which codes 6 structural proteins: N, F, P M, H and L.
Currently there are several variances in the nucleotide sequences
of N, F, M and H genes across wild type measles viruses, hence
measles viruses can be categorized into clades and genotypes. The
antigenicity of the previous genotype of measles is different from
the current genotype.
Objective To determine the antigenic differences between wild
type measles virus and measles vaccine virus.
Methods Analysis of the antigenic differences between wild type
virus (G2, G3 and D9) and vaccine virus (CAM-70 and Schwarz)
was performed by immunizing mice with the respective viruses.
The serum was then tested with micro-cross-neutralization
technique using the G2, G3, D9 and CAM-70 virus. Tests with
cross ELISA examination technique were also performed using
the same set of virus.
Results Analysis of the cross neutralization test and cross ELISA
showed that the highest antigenicity reaction was found between
wild type virus with antibody against wild type virus, while the
lowest reaction was between wild type virus with antibody against
CAM-70.
Conclusions We conclude that the antigenicity of antigenic protein
from wild type virus is higher than antigenicity of vaccine virus
protein. In addition, it was found that the antigenicity of proteins
from Schwarz vaccine virus was higher than proteins CAM-70
vaccine virus.
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F, Omilabuc SA, et al. Corresponding Author Contact
Information. Genotype and antigenic characterization of
hemaglutinin proteins of African measles virus isolates. Virus
Res 1999;62:89-95.
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detected in outbreacksin Japan after 2000. J Med Viral
2003;70:642-8.
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AIK-C strain expressing current wild-type hemagglutinin
protein. Vaccine 2004;22:309-16.
28. Xu W, Tamin A, Rota JS, Zhang L, Bellini WJ, Rota PA.
New genetic group of measles virus isolated in the People's
Republic of China. Virus Res 1998;54:147-56.
29. Sheshberadaran H, Norrby E. Characterization of epitopes on
the measles virus hemaglutinin. Virology 1986;152:58-65.
30. LiJina, Knowlesa WA, Rotab PA, Bellinib WJ, Browna DWG.
Genetic and antigenic characterization of the hemagglutinin
protein of measles virus strains recently circulating in the UK.
Virus Res 1998;55:1071-83.
31. Fournier P, Brons NH, Berbers GA, Wiesmiiller KH,
Fleckenstein BT, Schneider F, et al. Antibody to a new linear
site at the topographycal or fungtional interface between the
hemagglutinin and fusion protein protect against encephalitis.
J Gen Virol1997;78: 1295-1302.
ed. Philadelpia-New York: Lippincott-Raven; 1996. p. 1267-1312.
2. World Health Organization. Expanded program on
Immunization. Standardization of the nomenclature for
describing the genetic characterization of wild-type measles
viruses. Weekly Epidemiological report 2001;76:241-8.
3. Litbangkes DepKes RI: Laporan hasil genotipe virus campak
yang dikirim oleh WHO. 2002.
4. Setiawan M. Genotipe virus campak dan manfaatnya. 2007
(Submitted)
5. Bellini WJ, Rota JS, Rota PA. Virology of measles virus. J
Infect Dis 1994; 170:S 15-23.
6. Tamin A, Rota PA, Wang Z, Heath JL, Anderson, LJ, Bellini
WJ. Antigenic analysis of current wild type and vaccine
strains of measles virus. J Infect Dis 1994;170:795-801.
7. Atkinson WL, Orenstein W A. The resurgence of measles in
the United State, 1989-1990. Annu Rev Med 1992;43:451-
61.
8. Litbangkes DepKes RI: Laporan hasil pengamatan KLB di
beberapa wilayah di Indonesia. 1998
9. Gellin BG, Katz SL. Measles: state of the art and future
directions. J Infect Dis 1994; 170:S3-14.
10. RotaJS, HummelKB, Rota PA, Bellini WJ. Genetic variability
of the glycoprotein gene of current wild-type measles isolates.
Virology 1992;188:135-42.
11. Rota J, Rota P. Bellini W. Isolation and identification of
measles virus in cell culture: Manual revised July 24, 2002
for WHO/SEARO Inter-Country Training Course, Bangkok,
August 2002.
12. Madiyono B, Moeslichan Mz, Sastroasmoro S, Budiman I,
Purwanto H. Perkiraan besar sampel. In: Sastroasmoro S,
Ismael S, editors. Dasar-dasar metodologi penelitian klinis.
Jakarta: Binarupa Aksara; 1995. p. 187-212.
13. Pratiknya, A.W. Dasar-dasar metodologi penelitian
kedokteran dan kesehatan. 1"' Ed. Jakarta: Penerbit CV
Rajawali; 1986.
14. Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM,
Strober W. Current Protocols in Immunology. Vol. I.
Philadelphia: Current Protocols Wiley; 1996.
15. World Health Organization. Manual for the laboratory
diagnosis of measles virus infection. GENEVA: Departemen
of vaccines and biologicals; 1999.
16. Lannete EH, Schmid NJ. Diagnostic procedures for viral and
rickettsial infections; 4rh ed. New York: AVO Broadway;
1969. p. 116-8.
17. Pamungkas J, Iskandriati D, Sajuthi D, Grant RE Pembuatan
kit serologis enzyme immunoassay dan Westernblot
untuk pendeteksian anti SRV (Simian Retrovirus). Pusat
Studi Satwa Primata. Bogor: Lembaga Penelitian-Institut
Pertanian Bogor; 2003.
18. Iskandriati D. Identifikasi dan karakterisasi simian retrovirus
tipe-D pad a macaca fascicukaris, M.nemestrina dan presbitis
SPP [Dissertation]. Bogar: Institut Pertanian Bogar;
2004.
19. Hu A, Sheshberandaran H, Norbby E, Kovamees J.
Moleculer characterization of epitope on the measles virus
hemagglutinin protein. Virology 1993;192:351-4.
20. Setiawan M, Syahrurochman A, Ibrahin F, Swandono A.
Perbedaan sikuens gen protein H antara virus campak liar
dan virus vaksin campak di Indonesia. 2005 (Submitted).
21. Setiawan M, Syahrurochman A, Ibrahin F, Swandono A.
Perbedaan sikuens gen protein F antara virus campak liar
dan virus vaksin campak di Indonesia. 2005 (Submitted).
22. S Deroo, KC El Kasmi, P Fournier, D Theisen, NH Brons, M
Herrmann, et al. Enhanced antigenicity of a four-contactresidue
epitope of the measles virus hemagglutinin protein
by phage display libraries: evidence of helical structure in the
putative active site. Molecular Imunnol1998;35:435-43.
23. Rota PA, Liffick S, Rosenthal S, Heryanto B. Measles genotype
in Indonesia and Malaysia. Lancet 2000;355: 1557-8.
24. Birrer MJ, Udem S, Nathanson S, Bloom BR. Antegenic
variants of measles virus. Nature 1981;293:67-9.
25. Truonga AH, Kreisa S, Ammerlaana W, Harttera HK, Adu
F, Omilabuc SA, et al. Corresponding Author Contact
Information. Genotype and antigenic characterization of
hemaglutinin proteins of African measles virus isolates. Virus
Res 1999;62:89-95.
26. Zhou J, Fujino M, Inou Y. Genotype of measles virus was
detected in outbreacksin Japan after 2000. J Med Viral
2003;70:642-8.
27. Kumada A, Komase K, Nakayama T. Recobinant measles
AIK-C strain expressing current wild-type hemagglutinin
protein. Vaccine 2004;22:309-16.
28. Xu W, Tamin A, Rota JS, Zhang L, Bellini WJ, Rota PA.
New genetic group of measles virus isolated in the People's
Republic of China. Virus Res 1998;54:147-56.
29. Sheshberadaran H, Norrby E. Characterization of epitopes on
the measles virus hemaglutinin. Virology 1986;152:58-65.
30. LiJina, Knowlesa WA, Rotab PA, Bellinib WJ, Browna DWG.
Genetic and antigenic characterization of the hemagglutinin
protein of measles virus strains recently circulating in the UK.
Virus Res 1998;55:1071-83.
31. Fournier P, Brons NH, Berbers GA, Wiesmiiller KH,
Fleckenstein BT, Schneider F, et al. Antibody to a new linear
site at the topographycal or fungtional interface between the
hemagglutinin and fusion protein protect against encephalitis.
J Gen Virol1997;78: 1295-1302.
Published
2016-09-26
How to Cite
1.
Setiawan M, Sjahrurachman A, Ibrahim F, Suwandono A. Antigenic differences between wildtype measles viruses and vaccine viruses in Indonesia. PI [Internet]. 26Sep.2016 [cited 25Apr.2024];48(3):125-5. Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/683
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Received 2016-09-24
Accepted 2016-09-24
Published 2016-09-26
Accepted 2016-09-24
Published 2016-09-26
