Differences of antigenic profiles on immunoblotting of wild type measles virus and vaccine virus in Indonesia
Keywords:
measles virus, wild type virus, CAM-70 vaccine virus
Abstract
Background Measles virus has a negative, single strand RNAgenome which codes for six important structural proteins. The
genes of the wild type measles virus have many variances hence
the nucleotide sequences of each wild type virus and vaccine virus
are different. This differences lead to the antigenic differences
between wild type and vaccine virus.
Objective The purpose of this research is to investigate the
differences in the antigenic profiles on immunoblotting between
wild type and vaccine virus.
Results The analysis results are 1) the antigen ofCAM-70 vaccine
virus was less able in cross reacting with the antibodies from G2,
G3, 09, CAM-70 and Schwarz; 2) The antibody aga inst CAM-
70 was only able to cross react with antigens of N protein and a
few of antigens ofF proteins; 3) The wild type virus were very
immunogenic, hence the antibody titers were very high; 4) The
CAM-70 and MMR vaccine virus were less immunogenic, hence
their antibody were very low; 5) The antibody responses that
always occurred from all immunized mice serum were antibody
for N and F proteins. However, the antibody against CAM-70
vaccine virus was still able to react with wild type virus (G2, G3
and 09).
Conclusion All antigen-antibody reaction on immunoblotting
resulted in different profiles especially between wild type virus
and CAM-70 vaccine virus. Although CAM-70 vaccine virus
showed clear differences compared to G2, G3 and 09 genotypes,
antibodies against CAM-70 were still able to cross react with
antigens from other genotypes (G2, G3 and D9).
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detected in outbreacks in Japan after 2000. J Med Virol.
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WJ. Antigenic analysis of current wild type and vaccine
strains of measles virus. J Infect Dis. 1994;170:795-801.
24. Pratiknya A W. Dasar-dasar metodologi penelitian kedokteran
dan kesehatan. 1st ed. Jakarta: CV Rajawali; 1986.
Infct Dis. 1994;170:515-23.
2. WHO. Expanded program on immunization: Standardization
of the nomenclature for describing the genetic characteri-
zation of wild-type measles viruses. Weekly Epidemiological
Report. 1998;73:265-9.
3. Subdit Surveilans DirJen P2M dan PLP Departemen
Kesehatan Republik Indonesia: Data Laporan Tahunan
Penyakit Campak 2000-2002. Jakarta: Ministry of Health;
2003.
4. Litbangkes Departemen Kesehatan. Laporan hasil pengama-
tan KLB di beberapa wilayah di Indonesia. Jakarta: Ministry
of Health; 1998.
5. Tyrell DIJ, Norrby F. Structural polypeptides of measles virus.
J Gen Viral. 1978;39:219-29.
6. Rima BK. The proteins of morbilli viruses. J Gen Viral. 1983;
64:1205-19.
7. Griffin DE, and Bellini WJ. Measles Virus. In: Fields Virology.
3rd ed. Philadelpia-New York: Lippincott-Raven, 1996; p.
1267-1312.
8. WHO: Expanded program on immunization. Standardization
of the nomenclature foe describing the genetic characterization
of wild-type measles viruses. Weekly Epidemiological Report.
2001;76:241-8.
9. RotaJS, Hummel KB, Rota PA, Bellini WJ. Genetic variability
of the glycoprotein gene of current wild-type measles isolates.
VirolJ. 1992;188:135-42.
10. Rota JS, Wang ZD, Rota PA, Bellini WJ. Comparison of
sequnces of the H,F, and N coding genes of measles virus
vaccine strains. Virus Res. 1994;31:317-30.
11. Setiawan M. Genotipe virus campak dan manfaatnya. Ebers
Papyrus. 2006;12:143-56.
12. Rota J, Rota P, Bellini W. Isolation and identification of
measles virus in cell culture. Proceeding of the WHO/
SEARO Inter-Country Training Course; 2002 July 24;
Bangkok, Thailand. WHO; 2002.
13. Madiyono B, Muoeslichan Mz, Sastroasmoro S, Budiman I,
Purwanto H. Perkiraan besar sampel. In: Sastroasmoro S,
Ismael S. Dasar-dasar metodologi penelitian klinis. Jakarta:
Binarupa Aksara, 1995; p. 187-212.
14. Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM,
Strober W. Current protocols in immunology Vol. I National
Institute of Health. Wiley: Current Protocols Wiley; 1996.
15. Pamungkas J, Iskandriati D, Sajuthi D, Grant RF. Pembuatan
kit serologis enzyme immunoassay dan Westernblot untuk
pendeteksianantiSRV (Simian Retrovirus). Bogor: Pusatstudi
satwa primata lembaga penelitian-Institut Pertanian Bogor;
2003.
16. Iskandriati D. Identifikasi dan karakterisasi simian retrovirus
tipe-D pada macaca fascicularis, M.nemestrina dan presbitis
SPP [dissertation]. Bogar: Institut Pertanian Bogar; 2004.
17. Setiawan M, Syahrurochman A, Ibrahin F, Swandono A.
Perbedaan sikuens gen protein H antara virus campak
liar dan virus vaksin campak di Indonesia. Sari Pediatri.
2008;10:190-5.
18. Setiawan M, Syahrurochman A, Ibrahin F, Swandono A.
Perbedaan sikuens gen protein F antara virus campak liar
dan virus vaksin campak di Indonesia. Majalah Kedokteran
Indonesia. 2008;48:407 -12.
19. Setiawan M, Syahrurochman A, Ibrahin F, Swandono A.:
Perbedaan antigenisitas antara virus campak liar dan virus
vaksin campak di Indonesia. Paediatr Indones. 2008;48: 125-
35.
20. Birrer MJ, Udem S, Nathanson S, Bloom BR. Antegenic
variants of measles virus. Nature. 1981 ;293:67-69.
21. Truong AT, Kreis S, Ammerlaan W. Genotype and antigenic
characterization ofhemaglutinin proteins of African measles
virus isolates. Virus Res. 1999;62:89-95.
22. Zhou J, Fujino M, Inou Y. Genotype of measles virus was
detected in outbreacks in Japan after 2000. J Med Virol.
2003;70:642-8.
23. Tamin A, Rota PA, Wang Z, Heath JL, Anderson LJ, Bellini
WJ. Antigenic analysis of current wild type and vaccine
strains of measles virus. J Infect Dis. 1994;170:795-801.
24. Pratiknya A W. Dasar-dasar metodologi penelitian kedokteran
dan kesehatan. 1st ed. Jakarta: CV Rajawali; 1986.
Published
2016-09-15
How to Cite
1.
Setiawan M, Sjahrurachman A, lbrahim F, Suwandono A. Differences of antigenic profiles on immunoblotting of wild type measles virus and vaccine virus in Indonesia. PI [Internet]. 15Sep.2016 [cited 20Apr.2024];48(6):364-3. Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/631
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Received 2016-09-14
Accepted 2016-09-14
Published 2016-09-15
Accepted 2016-09-14
Published 2016-09-15
