Reverse Transcription Polymerase Chain Reaction (RT-PCR) as an influenza diagnostic test among children in Yogyakarta

  • Eva Musdalifah
  • M. Juffrie
  • Purnomo S
  • Amalia Amalia
Keywords: influenza, PCR, diagnostic test

Abstract

Background Influenza virus type A, especially H5Nl subtype or
avian influenza, is a highly pathogenic agent that causes epidemic
in the world with high mortality. Most cases are preschool and
school children. Anti-viral drug is effective when given at early
phase. The gold standard for the diagnosis of influenza is viral
culture, which takes 2 to 10 days. A rapid and accurate diagnostic
test is needed to control further viral infection.
Objective To determine the accuracy of RT-PCR as a diagnostic
test for children with influenza compared with viral culture.
Methods A cross-sectional study was conducted in primary health
cares of Jetis I, Godean I, II and Dr Sardjito Hospital Yogyakarta
between January 2005 and May 2007. The specimens, taken by
trained health personnel, were collected from both anterior nares
and throat of children aged from birth to 14 years who met the
eligibility criteria, then were stored in a frozen extraction tube
and sent to Jakarta for RT-PCR and viral culture as the gold
standard.
Results There were 34 7 children enrolled in this study. Influenza
infection was confirmed in 63 children (18.2%). There were 24
children with H3N2 subtype of influenza virus, 13 children with
HINI subtype, and one child with H5Nl subtype. The sensitivity,
specificity, positive and negative predictive value, positive and
negative likelihood ratio of RT-PCR test were 89%, 90%, 67%,
97%, 9, 3, 0, and 12 respectively.
Conclusions RT-PCR is accurate enough as influenza diagnostic
test in children.

Author Biographies

Eva Musdalifah
Department of Child Health, Medical School, Gadjah Mada
University, Yogyakarta, Indonesia.
M. Juffrie
Department of Child Health, Medical School, Gadjah Mada
University, Yogyakarta, Indonesia.
Purnomo S
Department of Child Health, Medical School, Gadjah Mada
University, Yogyakarta, Indonesia.
Amalia Amalia
Department of Child Health, Medical School, Gadjah Mada
University, Yogyakarta, Indonesia.

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Published
2016-09-15
How to Cite
1.
Musdalifah E, Juffrie M, S P, Amalia A. Reverse Transcription Polymerase Chain Reaction (RT-PCR) as an influenza diagnostic test among children in Yogyakarta. PI [Internet]. 15Sep.2016 [cited 29Mar.2024];48(5):288-1. Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/608
Received 2016-09-12
Accepted 2016-09-12
Published 2016-09-15