Translocation ETS leukemia-acute myeloid leukemia 1 (TEL-AML1) gene fusion in childhood acute lymphoblastic leukemia
Keywords:
acute lymphoblastic leukemia-TEL-AML1
Abstract
Background Acute lymphoblastic leukemia (ALL) in childrenis a heterogeneous disease with different subtypes based on their cellular and molecular characteristics. This condition would
influence the treatment outcome and subsequent risk for relapse. Accurate assignment of individual patients to risk groups is a critical issue for better outcome. TEL-AML1 gene fusion is the
most frequent in childhood ALL.
Objective The aim of this study was to investigate the incidence
ofTEL-AML1 children with ALL in Sardjito Hospital.
Methods This was a cross sectional study. In this preliminary
study, we used nested reverse-transcriptase polymerase chain
reaction (RT-PCR) to analyze the present of TEL-AML1 gene
fusion in bone marrow sample of childhood ALL patients.
Results We analyzed 41 samples. Out of these, 30 (73%) were
amplified. Twenry three out of 30 ALL patients with good medical
record were analyzed for this gene fusion. Out of 30 patients, there were five patients (17%) with TEL-AML1-positive gene fusion and 25 (83%) were TEL-AML1-negative. Among five patients with TEL-AML1-positive gene fusion, four patients (80%) were one year to less than 10 year old. All of the patients (100%) were with leukocyte < 50x109/L.
Conclusions TEL-AML1 gene fusion was found in 17 % of
samples. This gene fusion was more frequent in standard risk group (based on age and leukocyte). These data must be clarified with more samples. RT-PCR must be apply in all center as one part of improving diagnostic quality, especially in managing leukemia patients.
References
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fusion transcripts in children with acute lymphoblastic
leukemia, Singapore Med J. 2004;44:51 7-20.
2. Borkhardt G, Cazzaniga S, Viehmann MG, Valsecchi WD,
Ludwig L, Burci S, et al. Incidence and clinical relevance of
TEL-AML1 fusion genes in children with acute lymphoblastic
leukemia enrolled in the German and Italian multicenter
therapy trials. Blood. 1997;90:571- 7.
3. Downing JR, Shannon KM. Acute leukemia: a pediatric
perspective. Cancer Cell. 2002.;2:437-45
4. Fears S, Vignon C, Bohlander SK, Smith S, Rowley JD,
Nucifora G. Correlation between the ETV6/ CBFA2 (TEL-AML1) fusion gene and karyotypic abnormalities in children
with B-ee!! precursor acute lymphoblastic leukemia. Genes,
Chromosomes and Cancer .1996;17: 127-35.
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JB, Steinherz PG, et al. Frequency and clinical significance
of cytogenetic abnormalities in pediatric Tlineage acute
lymphoblastic leukemia: a report from the Children's Cancer
Group. J Clinical Oneal. 1998; 16:1270-8.
7. Liang DC, Shih LY, Yang CP, Hung I), Chen SH, )aing TH,
eta!. Multiplex RTPCR Assay for the Detection of Major
Fusion Transcripts in Taiwanese Children with B-Lineage
Acute Lymphoblastic Leukemia. Pediatr Oneal. 2002;
39:12-7.
8. Martinez-Climet JA. Molecular cytogenetics of childhood
hematological malignancies. Leukemia. 1997; 11: 1999-
2021.
9. Mulatsih S, Sutaryo. The result of treating children's acute
lymphoblastic leukemia (ALL) in Dr. Sardjito Hospital with
WK-ALL protocol 1999-2002. Bulletin Ilmu Kesehatan
Anak. 2005;17:808-19.
10. Nakao M, Yokota S, Horiike S. Detection and quantification
of TEL! AML 1 fusion transcripts by polymerase chain reaction
in childhood acute lymphoblastic leukemia. Leukemia. 1996;
10: 1463-70.
11. Pakakasama S, Kajanachumpol S, Kanjanapongkul S,
Sirachainan N, Meekaewkunchorn A, Ningsanond V, et al.
Simple multiplex RT-PCR for identifying common fusion
transcripts in childhood acute leukemia. lnt J Lab Hem.
2008;30:286-91.
12. Pui CH, Campana D, Evans WE. Childhood acute
lymphoblastic leukaemia-current status and future
perspectives. Lancet Oncol. 2001; 2:597-607.
13. Pui CH, Evans WE. Acute lymphoblastic leukemia. N Eng!
J Med. 1998;339:605-15.
14. Romana SP, Mauchaufee M, Coniat ML, Chumakov I, Pastier
DL, Berger R, et al. The t (12; 21) of acute lymphoblastic
leukemia results in a tel-AML1 gene fusion. Blood.
1995;85:3662-70.
15. Rubnitz JR, Behm FG, Ryan C, Sandlund JT, Ribeiro RC,
Rivera GK, et al. Low frequency of TEL-AML1 in relapsed
acute lymphoblastic leukemia supports a favorable prognosis
for this genetic subgroup. Leukemia. 1999;13:19-21.
16. Shurtleff SA, Buijs A, Behm FG, Rubnitz JE, Raimondi SC,
Hancock ML, .TEL-AML1 fusion resulting from a cryptic
t (12; 21) is the most common genetic lesion in pediatric
ALL and defines a subgroup of patients with an excellent
prognosis. Leukemia. 1995;9: 1985-9.
multiplex RTPCR assay for screening significant oncogene
fusion transcripts in children with acute lymphoblastic
leukemia, Singapore Med J. 2004;44:51 7-20.
2. Borkhardt G, Cazzaniga S, Viehmann MG, Valsecchi WD,
Ludwig L, Burci S, et al. Incidence and clinical relevance of
TEL-AML1 fusion genes in children with acute lymphoblastic
leukemia enrolled in the German and Italian multicenter
therapy trials. Blood. 1997;90:571- 7.
3. Downing JR, Shannon KM. Acute leukemia: a pediatric
perspective. Cancer Cell. 2002.;2:437-45
4. Fears S, Vignon C, Bohlander SK, Smith S, Rowley JD,
Nucifora G. Correlation between the ETV6/ CBFA2 (TEL-AML1) fusion gene and karyotypic abnormalities in children
with B-ee!! precursor acute lymphoblastic leukemia. Genes,
Chromosomes and Cancer .1996;17: 127-35.
5. Hariyana SM. The importance of molecular biology studies
of clinical leukemia in Indonesia. Proceeding of the Scientific
Seminar on Leukemia; 1998 September 26; Yogyakarta,
Indonesia.
6. Heerema NA, Sather HN, Sensei MG, Kraft P, Nachman
JB, Steinherz PG, et al. Frequency and clinical significance
of cytogenetic abnormalities in pediatric Tlineage acute
lymphoblastic leukemia: a report from the Children's Cancer
Group. J Clinical Oneal. 1998; 16:1270-8.
7. Liang DC, Shih LY, Yang CP, Hung I), Chen SH, )aing TH,
eta!. Multiplex RTPCR Assay for the Detection of Major
Fusion Transcripts in Taiwanese Children with B-Lineage
Acute Lymphoblastic Leukemia. Pediatr Oneal. 2002;
39:12-7.
8. Martinez-Climet JA. Molecular cytogenetics of childhood
hematological malignancies. Leukemia. 1997; 11: 1999-
2021.
9. Mulatsih S, Sutaryo. The result of treating children's acute
lymphoblastic leukemia (ALL) in Dr. Sardjito Hospital with
WK-ALL protocol 1999-2002. Bulletin Ilmu Kesehatan
Anak. 2005;17:808-19.
10. Nakao M, Yokota S, Horiike S. Detection and quantification
of TEL! AML 1 fusion transcripts by polymerase chain reaction
in childhood acute lymphoblastic leukemia. Leukemia. 1996;
10: 1463-70.
11. Pakakasama S, Kajanachumpol S, Kanjanapongkul S,
Sirachainan N, Meekaewkunchorn A, Ningsanond V, et al.
Simple multiplex RT-PCR for identifying common fusion
transcripts in childhood acute leukemia. lnt J Lab Hem.
2008;30:286-91.
12. Pui CH, Campana D, Evans WE. Childhood acute
lymphoblastic leukaemia-current status and future
perspectives. Lancet Oncol. 2001; 2:597-607.
13. Pui CH, Evans WE. Acute lymphoblastic leukemia. N Eng!
J Med. 1998;339:605-15.
14. Romana SP, Mauchaufee M, Coniat ML, Chumakov I, Pastier
DL, Berger R, et al. The t (12; 21) of acute lymphoblastic
leukemia results in a tel-AML1 gene fusion. Blood.
1995;85:3662-70.
15. Rubnitz JR, Behm FG, Ryan C, Sandlund JT, Ribeiro RC,
Rivera GK, et al. Low frequency of TEL-AML1 in relapsed
acute lymphoblastic leukemia supports a favorable prognosis
for this genetic subgroup. Leukemia. 1999;13:19-21.
16. Shurtleff SA, Buijs A, Behm FG, Rubnitz JE, Raimondi SC,
Hancock ML, .TEL-AML1 fusion resulting from a cryptic
t (12; 21) is the most common genetic lesion in pediatric
ALL and defines a subgroup of patients with an excellent
prognosis. Leukemia. 1995;9: 1985-9.
Published
2009-10-31
How to Cite
1.
Mulatsih S, Liang Y, Yeoh A, Sutaryo S, Sunarto S. Translocation ETS leukemia-acute myeloid leukemia 1 (TEL-AML1) gene fusion in childhood acute lymphoblastic leukemia. PI [Internet]. 31Oct.2009 [cited 13Nov.2024];49(5):270-. Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/582
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Received 2016-09-11
Accepted 2016-09-11
Published 2009-10-31
Accepted 2016-09-11
Published 2009-10-31
