Difference of hemaglutinins between wild-type and vaccine measles virus in Indonesia
Keywords:
measles, wilde-type, vaccine-type, hemagglutinin gene, hemagglutinin protein
Abstract
Background Hemaglutinin (H) protein of measles virus is veryimportant in the process of host cell infection. H protein is also
able to induce specific antibodies which can neutralize measles
virus and block the cell infection.
Objective This study aimed to explore the nucleotide and amino
acid sequence differences between wild-type measles virus (G2,
G3 and D9) with CAM-70, Schwarz and Edmonston-wt vaccine
virus.
Methods The exctration and amplification of the gene were
conducted in the laboratory using biomolecular technology. The
gene and protein analysis were conducted using the bioinformatic
technology.
Results The results showed that the differences in nucleotide
sequences were highest between wild-type virus and CAM-70
vaccine virus (76-77 nucleotides), followed by Schwarz (61-64
nucleotides) and Edmonston (60-63 nucleotides). The differences
in amino acid sequences were highest between wild-type virus
and CAM-70 (24-29 residues), followed by Schwarz (13-20
residues) and Edmonston (12-19 residues).
Conclusion The Indonesian wild-type measles virus was genetically
closer to Schwarz vaccine virus than CAM-70 vaccine virus,
hence the neutralizing antibodies generated by Schwarz vaccine
were more specific against Indonesian wild-type virus compared
to CAM-70 vaccine.
References
1. Nelson JD, Sandusky G, Peck FB. Measles skin test and
serologic respons to intradermal measles antigen. JAMA
1966;198:185-6.
2. Fulginiti VA, ArthurJH. Altered reactivity to measles virus.
J Pediatr 1969;75:609-16.
3. Parks CL, Lerch RA, Walpita P, Wang HP, Sidhu MS, Udem
SA. Comparison of predicted amino acid sequences of
measles virus strains in the Edmonston vaccine lineage. J
Virol 2001;75:910-20.
4. Tyrell DIJ, Norrby F. Structural polypeptides of measles virus.
J Gen Virol 1978;39:219-29.
5. Rima BK. The proteins of morbilli viruses. J Gen Virol
1983;64:1205-19.
6. Griffin DE, Ward BJ, Esolen LM. Pathogesis of measles virus
infection: An hypothesis for altered immune responses. J Infec
Dis 1994;170: S24-31.
7. Rose JW, Bellini WJ, McFarlin DE, McFarland HF. Human
celluler immune response to measles virus polypeptides. J
Virol 1984;49:988-91.
8. World Health Organization. Expanded program on
immunization. Standardization of the nomenclature for
describing the genetic characterization of wild-type
measles viruses. Weekly Epidemiological report 2001;
76:241-8.
9. Tamin A, Rota PA, Wang Z, Heath JL, Anderson LJ, Bellini
WJ. Antigenic analysis of current wild type and vaccine
strains of measles virus. J Infect Dis 1994;170:795-801.
10. Bellini WJ, Rota JS, Rota PA. Virology of measles virus. J
Infect Dis 1994;170:S15-23.
11. Wong TC, Ayata M, Ueda S, Hirano A. Role of biased
hypermutation in evolution of subacute sclerosing
panencephlitis virus from progenitor acute measles virus. J
Virol 1991;65:2191-9.
12. Taylor MJ, Godfrey E, Baczko K, Ter meulen V, Wild TF,
Rima BK. Identification of several different lineages of
measles virus. J Gen Virol 1991;72:83-8.
13. Setiawan M. Analisis genetik dan antigenik virus campak
liar dan virus vaksin di Indonesia [dissertation]. Jakarta:
Fakultas Kedokteran Universitas Indonesia; 2005.
14. Komase K, Suzuki N, Nakayama T, Miki K, Kawanishi R,
Fukuda K. Genom sequence of measles virus. NCBI no.
accession: AB046218 (2001).
15. Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober
W. Current Protocols in Immunology Volume I. National Institute
of Health; Published by Current Protocols Wiley, (1996).
16. Aicardi J, Goutieres F, Arseneo-Nunes ML, Lebon P. Acute
measles encephalitis In children with immunosuppression.
Pediatrics 1977 59:232-9.
17. Beauverger P, Chadwick J, Buckland R, Wild TF.
Serotype-specific and canine distemper virus cross-
reactive H-2KK-restricted cytotoxic T lymphocyte
epitopes in the measles virus nucleoprotein. Virology
1994;203:172-7.
18. Hu A, Sheshberandaran H, Norbby E, Kovamees J. Moleculer
characterization of epitope on the measles virus hemagglutinin
protein. Virology 1993;192:351-4.
19. Obeid OE, Partidos CD, Steward MW. Analysis of the antigenic
profile of measles virus haemagglutinin in mice and humans
using overlapping synthetic peptides. Virus Res 1994;32:69-84.
serologic respons to intradermal measles antigen. JAMA
1966;198:185-6.
2. Fulginiti VA, ArthurJH. Altered reactivity to measles virus.
J Pediatr 1969;75:609-16.
3. Parks CL, Lerch RA, Walpita P, Wang HP, Sidhu MS, Udem
SA. Comparison of predicted amino acid sequences of
measles virus strains in the Edmonston vaccine lineage. J
Virol 2001;75:910-20.
4. Tyrell DIJ, Norrby F. Structural polypeptides of measles virus.
J Gen Virol 1978;39:219-29.
5. Rima BK. The proteins of morbilli viruses. J Gen Virol
1983;64:1205-19.
6. Griffin DE, Ward BJ, Esolen LM. Pathogesis of measles virus
infection: An hypothesis for altered immune responses. J Infec
Dis 1994;170: S24-31.
7. Rose JW, Bellini WJ, McFarlin DE, McFarland HF. Human
celluler immune response to measles virus polypeptides. J
Virol 1984;49:988-91.
8. World Health Organization. Expanded program on
immunization. Standardization of the nomenclature for
describing the genetic characterization of wild-type
measles viruses. Weekly Epidemiological report 2001;
76:241-8.
9. Tamin A, Rota PA, Wang Z, Heath JL, Anderson LJ, Bellini
WJ. Antigenic analysis of current wild type and vaccine
strains of measles virus. J Infect Dis 1994;170:795-801.
10. Bellini WJ, Rota JS, Rota PA. Virology of measles virus. J
Infect Dis 1994;170:S15-23.
11. Wong TC, Ayata M, Ueda S, Hirano A. Role of biased
hypermutation in evolution of subacute sclerosing
panencephlitis virus from progenitor acute measles virus. J
Virol 1991;65:2191-9.
12. Taylor MJ, Godfrey E, Baczko K, Ter meulen V, Wild TF,
Rima BK. Identification of several different lineages of
measles virus. J Gen Virol 1991;72:83-8.
13. Setiawan M. Analisis genetik dan antigenik virus campak
liar dan virus vaksin di Indonesia [dissertation]. Jakarta:
Fakultas Kedokteran Universitas Indonesia; 2005.
14. Komase K, Suzuki N, Nakayama T, Miki K, Kawanishi R,
Fukuda K. Genom sequence of measles virus. NCBI no.
accession: AB046218 (2001).
15. Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober
W. Current Protocols in Immunology Volume I. National Institute
of Health; Published by Current Protocols Wiley, (1996).
16. Aicardi J, Goutieres F, Arseneo-Nunes ML, Lebon P. Acute
measles encephalitis In children with immunosuppression.
Pediatrics 1977 59:232-9.
17. Beauverger P, Chadwick J, Buckland R, Wild TF.
Serotype-specific and canine distemper virus cross-
reactive H-2KK-restricted cytotoxic T lymphocyte
epitopes in the measles virus nucleoprotein. Virology
1994;203:172-7.
18. Hu A, Sheshberandaran H, Norbby E, Kovamees J. Moleculer
characterization of epitope on the measles virus hemagglutinin
protein. Virology 1993;192:351-4.
19. Obeid OE, Partidos CD, Steward MW. Analysis of the antigenic
profile of measles virus haemagglutinin in mice and humans
using overlapping synthetic peptides. Virus Res 1994;32:69-84.
Published
2008-02-29
How to Cite
1.
Setiawan M, Sjahrurachman A, Ibrahim F, Suwandono A. Difference of hemaglutinins between wild-type and vaccine measles virus in Indonesia. PI [Internet]. 29Feb.2008 [cited 22Nov.2024];48(1):42-. Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/465
Section
Articles
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Received 2016-09-05
Accepted 2016-09-05
Published 2008-02-29
Accepted 2016-09-05
Published 2008-02-29
