Antimicrobial activity of homemade WHO ethanol-based hand rub solution in pediatric department, Dr. Cipto Mangunkusumo National Referral Hospital
Abstract
Background Hand hygiene is essential in reducing healthcare-associated infections. Alcohol-based hand rub solutions have been reported to have superior antimicrobial efficacy on both bacteria and lipophilic viruses compared to washing with hand soap. In low- and middle-income countries, the cost of infection control poses a challenge. Our hospital produced an ethanol-based hand rub based on a WHO formulation to reduce the infection prevention costs.
Objectives To identify the antimicrobial activity of a WHO ethanol-based hand rub solution against bacterial contamination on the hands of healthcare workers at the Department of Child Health, Dr. Cipto Mangunkusumo National Referral Hospital.
Methods This cross-sectional study was performed on the hands of healthcare workers (physicians and nurses) working in the Department of Child Health, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta. A total of 225 specimens from 75 subjects were obtained by collecting swabs on both hands before and after participants worked in the Department of Child Health, Dr. Cipto Mangunkusumo General Hospital. Bacterial culture tests were performed to identify gram-positive and gram-negative bacteria. Bacteria were grouped into no-growth/Bacillus sp, Enterobacteriaceae, cocci, and non-fermenter groups.
Results The use of our WHO ethanol-based hand rub solution, generally resulted in a statistically significant decrease in bacterial growth from 84 to 54.6%, before compared to after the hand rub was performed. In more detail, there was a 72.7% decrease in Enterobacteriaceae, a 71.4% decrease in non-fermenters, an 8.6% decrease in cocci and a 44.1% increase in the number of specimens showing no growth bacteria/Bacillus sp.
Conclusion Our WHO ethanol-based hand rub has significant antimicrobial activity for common nosocomial pathogens (e.g., Staphylococcus aureus, P. aeruginosa, and K. pneumoniae).
References
2. Laustsen S, Lund E, Bibby BM, Kristensen B, Thulstrup AM, Kjolseth-Moller J. Effect of correctly using alcohol-based hand rub in a clinical setting. Infect Control Hosp Epidemiol. 2008;29:954-6. DOI: https://doi.org/10.1086/590393.
3. Brown SM, Lubimova AV, Khrustalyeva NM, Shulaeva SV, Tekhova I, Zueva LP, et al. Use of an alcohol-based handrub and quality improvement interventions to improve hand hygiene in a Russian neonatal intensive care unit. Infect Control Hosp Epidemiol. 2003;24:172-9. DOI: https://doi.org/10.1086/502186.
4. World Health Organization guidelines on hand hygiene in health care [Internet]. WHO Int. 2021 [cited 2021 September 8]. Available from: https://www.who.int/publications/i/item/9789241597906
5. Padsalg A, Jain D, Bidkar S, Harinarayana D, Jadhav V. Preparation and evaluation of handrub disinfectant. Asian J Pharm. 2008;2:18-21. DOI: https://doi.org/10.4103/0973-8398.41559.
6. Guide to local production: WHO-recommended handrub formulations. [Internet]. WHO.Int. 2021 [cited 2021 September 8]. Available from: https://www.who.int/gpsc/5may/Guide_to_Local_Production.pdf
7. Mathur P. Hand hygiene: back to the basics of infection control. Indian J Med Res. 2011;134:611-20. DOI: https://doi.org/10.4103/0971-5916.90985
8. Kampf G, Hollingsworth A. Comprehensive bactericidal activity of an ethanol-based hand gel in 15 seconds. Ann Clin Microbiol Antimicrob. 2008;7:2. DOI: https://doi.org/10.1186/1476-0711-7-2.
9. Oke MA, Bello AB, Odebisi MB, El-Imam AMA, Kazeem MO. Evaluation of antibacterial efficacy of some alcohol-based hand sanitizers sold in Ilorin (North-Central Nigeria). Ife J Sci. 2013;15:111-7.
10. Kumar D, Kaushal SK, Kumar G, Prakash V, Prakash P, Nath G. Evaluation of the antibacterial activity of commonly used alcohol-based hand sanitizers on common pathogenic bacteria. Indian J Appl Res. 2015;3:2249-555. DOI: https://doi.org/10.36106/ijar.
Copyright (c) 2022 Nina Dwi Putri, Hindra Irawan Satari, Mulya Rahma Karyanti, Ari Prayitno, Pratama Wicaksana, Anis Karuniawati, Delly Chipta Lestari, Nabila Maudy Salma, Shindy Claudya Aprianti, Amalia Almira, Andi Annisa Rusyda Khafiyani
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Accepted 2022-07-25
Published 2022-07-25
