Association between low-grade chronic inflammation with adipocytokines and body fat mass in superobese male children

Main Article Content

Aryono Hendarto
Sudigdo Sastroasmoro
Damayanti Rusli Sjarif

Abstract

Background Obesity causes adipocytokines dysregulation and enhances the pro-inflammatory response. Low-grade chronic inflammation is related to cardiometabolic diseases.


Objective To evaluate the status of low-grade chronic inflammation in pre-pubertal, obese boys and its potential correlation to adipocytokines and body fat mass.


Methods This cross-sectional study included pre-pubertal, male, superobese children as the subjects. We determined obesity status using the CDC 2000 BMI-for-age chart. Body fat percentage was measured using bioelectric impedance analysis (BIA). Fasting blood specimens were collected to evaluate hsCRP, leptin, adiponectin, and TNF-α levels.


Results Eighty subjects were recruited into this study, with a mean age of 6.9 years. Ten subjects (12.5%) had low-grade chronic inflammation (hsCRP level ≥ 1 mg/L). The levels of hsCRP was not correlated with leptin, adiponectin, and TNF-α levels. A weak, but significant correlation was observed between hsCRP level and body fat mass (r= +0.383; P<0.0001). The hsCRP level increased with increasing body fat mass, until it reached its peak at body fat mass of 28 kg. Beyond that point, hsCRP level was stable.


Conclusion Low-grade chronic inflammation begins at a young age in obese children. The hsCRP level has a weak correlation with body fat mass, but no correlation with adipocytokine levels. Prevention and treatment of childhood obesity should be prioritized to prevent further cardiovascular and metabolic diseases.

Article Details

How to Cite
1.
Hendarto A, Sastroasmoro S, Sjarif D. Association between low-grade chronic inflammation with adipocytokines and body fat mass in superobese male children. PI [Internet]. 18Feb.2019 [cited 24Apr.2019];59(1). Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/2072
Section
Pediatric Nutrition & Metabolic Disease

References

1. Musaiger AO. Overweight and obesity in Eastern Mediterranean region: prevalence and possible causes. J Obes. 2011;2011:407237. https://doi.org/10.1155/2011/407237 PMid:21941635 PMCid:PMC3175401
2. Salehiniya H, Yazdani K, Barekati H, Asadi Lari M. The prevalence of overweight and obesity in children under 5 years in Tehran, Iran, in 2012: a population-based study. Res Cardiovasc Med. 2016;5:e30425. https://doi.org/10.5812/cardiovascmed.30425 PMid:26889459 PMCid:PMC4752595
3. Balitbang Kemenkes RI. Riset Kesehatan Dasar (RISKESDAS) 2013. Jakarta: Kemenkes RI; 2013. p.259.
4. Monteiro R, Azevedo I. Chronic inflammation in obesity and the metabolic syndrome. Mediators Inflamm. 2010;2010:289645. https://doi.org/10.1155/2010/289645 PMid:20706689 PMCid:PMC2913796
5. Engin A. Adipose tissue hypoxia in obesity and its impact on preadipocytes and macrophages: hypoxia hypothesis. Adv Exp Med Biol. 2017;960:305-26. https://doi.org/10.1007/978-3-319-48382-5_13 PMid:28585205
6. Iikuni N, Lam QLK, Lu L, Matarese G, Cava AL. Leptin and inflammation. Curr Immunol Rev. 2008;4:70-9. https://doi.org/10.2174/157339508784325046 PMid:20198122 PMCid:PMC2829991
7. Procaccini C, De Rosa V, Galgani M, Carbone F, La Rocca C, Formisano L, et al. Role of adipokines signaling in the modulation of T cells function. Front Immunol. 2013;4:332. https://doi.org/10.3389/fimmu.2013.00332 PMid:24151494 PMCid:PMC3799205
8. de Rooij SR, Nijpels G, Nilsson PM, Nolan JJ, Gabriel R, Bobbioni-Harsch E, et al. Low-grade chronic inflammation in the relationship between insulin sensitivity and cardiovascular disease (RISC) population: associations with insulin resistance and cardiometabolic risk profile. Diabetes Care. 2009;32:1295-301. https://doi.org/10.2337/dc08-1795 PMid:19366964 PMCid:PMC2699728
9. Seo HS. The role and clinical significance of high-sensitivity C-reactive protein in cardiovascular disease. Korean Circ J. 2012;42:151-3. https://doi.org/10.4070/kcj.2012.42.3.151 PMid:22493609 PMCid:PMC3318086
10. Parizkova J, Hills A. Childhood obesity prevention and treatment. 2nd ed. Florida: CRC Press; 2005. p.28.
11. Guran O, Akalin F, Ayabakan C, Dereli FY, Haklar G. High-sensitivity C-reactive protein in children at risk for coronary artery disease. Acta Paediatr. 2007;96:1214-9. https://doi.org/10.1111/j.1651-2227.2007.00377.x PMid:17655623
12. Divella R, De Luca R, Abbate I, Naglieri E, Daniele A. Obesity and cancer: the role of adipose tissue and adipo-cytokines-induced chronic inflammation. J Cancer. 2016;7:2346-59. https://doi.org/10.7150/jca.16884 PMid:27994674 PMCid:PMC5166547
13. Zhao D, Liu H. Adipose tissue dysfunction and the pathogenesis of metabolic syndrome. World J Hypertens. 2013;3:18-26. https://doi.org/10.5494/wjh.v3.i3.18
14. Kamath DY, Xavier D, Sigamani A, Pais P. High sensitivity C-reactive protein (hsCRP) & cardiovascular disease: an Indian perspective. Indian J Med Res. 2015;142:261-8. https://doi.org/10.4103/0971-5916.166582 PMid:26458341 PMCid:PMC4669860
15. Wang A, Liu J, Li C, Gao J, Li X, Chen S, et al. Cumulative exposure to high-sensitivity C-reactive protein predicts the risk of cardiovascular disease. J Am Heart Assoc. 2017;6:e005610. https://doi.org/10.1161/JAHA.117.005610 PMid:29066453 PMCid:PMC5721824
16. Cozlea DL, Farcas DM, Nagy A, Keresztesi AA, Tifrea R, Cozlea L, et al. The impact of C reactive protein on global cardiovascular risk on patients with coronary artery disease. Curr Health Sci J. 2013;39:225-31. PMid:24778862 PMCid:PMC3945266
17. Shrivastava AK, Singh HV, Raizada A, Singh SK. C-reactive protein, inflammation and coronary heart disease. Egypt Heart J. 2015;67:89-97. https://doi.org/10.1016/j.ehj.2014.11.005
18. Ekmen N, Helvaci A, Gunaldi M, Sasani H, Yildirmak ST. Leptin as an important link between obesity and cardiovascular risk factors in men with acute myocardial infarction. Indian Heart J. 2016;68:132-7. https://doi.org/10.1016/j.ihj.2015.07.032 PMid:27133319 PMCid:PMC4867951
19. Lam QL, Zheng BJ, Jin DY, Cao X, Lu L. Leptin induces CD40 expression through the activation of Akt in murine dendritic cells. J Biol Chem. 2007;282:27587-97. https://doi.org/10.1074/jbc.M704579200 PMid:17660512
20. Francisco V, Pino J, Campos-Cabaleiro V, Ruiz-Fernandez C, Mera A, Gonzalez-Gay MA, et al. Obesity, fat mass and immune system: role for leptin. Front Physiol. 2018;9:640. https://doi.org/10.3389/fphys.2018.00640 PMid:29910742 PMCid:PMC5992476
21. Nakahara K, Okame R, Katayama T, Miyazato M, Kangawa K, Murakami N. Nutritional and environmental factors affecting plasma ghrelin and leptin levels in rats. J Endocrinol. 2010;207:95-103. https://doi.org/10.1677/JOE-10-0062 PMid:20631048
22. Sertoglu E. Importance of factors affecting serum leptin levels. World J Surg. 2015;39:1587-8. https://doi.org/10.1007/s00268-014-2931-2 PMid:25561198
23. Brydon L. Adiposity, leptin and stress reactivity in humans. Biol Psychol. 2011;86:114-20. https://doi.org/10.1016/j.biopsycho.2010.02.010 PMid:20193730 PMCid:PMC3042594
24. Ouchi N, Walsh K. Adiponectin as an anti-inflammatory factor. Clin Chim Acta. 2007;380:24-30. https://doi.org/10.1016/j.cca.2007.01.026 PMid:17343838 PMCid:PMC2755046
25. Butte NF, Comuzzie AG, Cai G, Cole SA, Mehta NR, Bacino CA. Genetic and environmental factors influencing fasting serum adiponectin in Hispanic children. J Clin Endocrinol Metab. 2005;90:4170-6. https://doi.org/10.1210/jc.2004-2328 PMid:15827100
26. Kotani K, Sakarie N, Saiga K, Kato M, Ishida K, Kato Y, et al. Serum adiponectin levels and lifestyle factors in Japanese men. Heart Vessels. 2007;22:291-6. https://doi.org/10.1007/s00380-006-0969-2 PMid:17879019
27. Tzanavari T, Giannogonas P, Karalis KP. TNF-alpha and obesity. Curr Dir Autoimmun. 2010;11:145-56. https://doi.org/10.1159/000289203 PMid:20173393
28. Page MJ, Bester J, Pretorius E. The inflammatory effects of TNF-α and complement component 3 on coagulation. Sci Rep. 2018;8:1812. https://doi.org/10.1038/s41598-018-20220-8 PMid:29379088 PMCid:PMC5789054
29. Indulekha K, Surendar J, Mohan V. High sensitivity C-reactive protein, tumor necrosis factor-α, interleukin-6, and vascular cell adhesion molecule-1 levels in Asian Indians with metabolic syndrome and insulin resistance (CURES-105). J Diabetes Sci Technol. 2011;5:982-8. https://doi.org/10.1177/193229681100500421 PMid:21880241 PMCid:PMC3192605
30. Gokulakrishnan K, Deepa R, Mohan V. Association of high sensitivity C-reactive protein (hsCRP) and tumour necrosis factor-alpha (TNF-alpha) with carotid intimal medial thickness in subjects with different grades of glucose intolerance--the Chennai Urban Rural Epidemiology Study (CURES-31). Clin Biochem. 2008;41:480-5. https://doi.org/10.1016/j.clinbiochem.2008.01.030 PMid:18328265
31. Tangvarasittichai S, Pongthaisong S, Tangvarasittichai O. Tumor necrosis factor-Α, interleukin-6, C-reactive protein levels and insulin resistance associated with type 2 diabetes in abdominal obesity women. Indian J Clin Biochem. 2016;31:68-74. https://doi.org/10.1007/s12291-015-0514-0 PMid:26855490 PMCid:PMC4731374
32. Castoldi G, Galimberti S, Riva C, Papagna R, Querci F, Casati M, et al. Association between serum values of C-reactive protein and cytokine production in whole blood of patients with type 2 diabetes. Clin Sci (Lond). 2007;113:103-8. https://doi.org/10.1042/CS20060338 PMid:17362204
33. Fuentes E, Fuentes F, Vilahur G, Badimon L, Palomo I. Mechanisms of chronic state of inflammation as mediators that link obese adipose tissue and metabolic syndrome. Mediators Inflamm. 2013;2013:136584. https://doi.org/10.1155/2013/136584 PMid:23843680 PMCid:PMC3697419