Probiotic Weisella paramesenteroides on enteropathogenic E. coli-induced diarrhea

Main Article Content

Aslinar Aslinar
Yusri Dianne Jurnalis
Endang Purwati
Yorva Sayoeti

Abstract

Background


Enteropathogenic Escherichia coli (EPEC) is a causative agent of intestinal inflammation and microfloral imbalance, leading to diarrhea. The presence of tumor necrosis factor-α (TNF-α) in the feces is an indicator of inflammation in the intestinal mucosa. Dadih, (local made of fermented buffalo milk), contains probiotics and is widely consumed by the people in West Sumatera, Indonesia. Weisella paramesenteroides, a probiotic lactic acid bacteria (LAB), has been isolated from dadih and is believed to be useful for improving intestinal microflora balance and inhibiting the activity of harmful microbes.


Objective


To determine the efficacy of W. paramesenteroides administration in various doses and durations on bowel frequency, stool’s TNF-α levels, and intestinal microflora balance on mice with EPEC-induced diarrhea.


Method


This randomized experimental animal study examined two factors relating to the effects of W. paramesenteroides on EPEC-induced diarrhea, namely doses of probiotics (factor A), and durations of observation (factor B). The subjects consisted of 100 male white mice (Mus musculus) aged 8 weeks, with weights of 25-30 grams. The outcomes measured were bowel frequency, stool’s TNF-α levels, and the balance of intestinal microflora on mice with EPEC-induced diarrhea.  Subjects were divided into 5 groups: the negative control group (received neither EPEC nor probiotic), positive control group (received only EPEC), and three experimental groups (received EPEC and different doses of W. paramesenteroides ). Probiotics were given twice at the 12-hours and 24-hours for the experimental groups, while the durations of observation conssited of baseline, 12 hours, 24 hours, and 36 hours.


Results


After 36 hours, subjects with EPEC-induced diarrhea who received W. Paramesenteroides administration in doses of 2 x 108 (A3), were found to have the largest decline of mean defecation (a 4.4-fold decline) and the largest decline of stool’s mean TNF-α levels (48.3 pg/mL), compared to the positive control group, and other experimental groups who received higher doses of probiotics.

Article Details

How to Cite
1.
Aslinar A, Jurnalis Y, Purwati E, Sayoeti Y. Probiotic Weisella paramesenteroides on enteropathogenic E. coli-induced diarrhea. PI [Internet]. 28Feb.2014 [cited 21Sep.2019];54(1):1-. Available from: https://paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/159
Section
Articles
Received 2016-08-15
Accepted 2016-08-15
Published 2014-02-28

References

1. WHO, UNICEF. Diarrhoea: why children are still dying and what can be done. 2009.
2. Subagyo B, Santosa NB. Diare Akut. In: Juffrie M SS, Oswari H, Arief S, Roasalina I, Mulyani NS, et al ed. Buku Ajar Gastroenterologi-Hepatologi. Jakarta: IDAI; 2010. p. 87-120
3. Thapar N. Diarrhoea in children: an interface between developing and developed countries. Lancer. 2004;363:641-53
4. Kelleher Sl, Casas I, Carbajal N, Lonnerdal B. Supplementation of infant formula with the probiotic Lactobacillus reuteri and zinc: impact on enteric infection and nutrition in infant rhesus monkeys. J Pediatr Gastroenterol Nutr. 2002;35:162-8.
5. Karuniawati. Pengaruh suplementasi seng dan probiotik terhadap durasi diare cair anak [tesis]. Semarang: Universitas Diponegoro; 2010.
6. Rosalina I. Efikasi pemberian zinc pada diare. In: Naskah lengkap Konas III Badan Koordinasi Gastroenterologi Anak Indonesia; 2007 December 6-8. Surabaya; 2007. p 250-3.
7. Collado MC< Isolauri E, Salminen S, Sanz Y. The impact of probiotic on gut health. Current Drug Metabolism. 2009; 10:68-78.
8. Pena JA, Versalovic J. Lactobacillus rhamnosus GG decreases TNF-alpha production in lypopolysaccharide-activated murine macrophages by a contact-independent mechanism. Cellular Microbiol. 2003;5:277-85
9. Ng SC, Hart AL, Kamm MA, Stagg AJ, Knight SC. Mechanism of action of probiotics: recent advance. Inflamm Bowel Dis. 2009;15:300-10.
10. Goel AJ, Dilbaghi N, Kamboj DV, Sing L. Probiotics: microbial therapy for health modulation. Defence Sci J. 2006;56:513-29.
11. Narayan SS, Jalgaonkar S, Shahani S, Kulkarni UN. Probiotics: current trends in the treatment of diarrhea. Hongkong Med J. 2010;16:213-8.
12. Purwati E, Arif, Rahmadi A. Buku ajar teknologi dadih. Bogor: Cendikia Publ House; 2011. p. 80-133.
13. Papagianni M, Papamichael EM. Purification, amino acid sequence and characterization of the class Ila bacteriocinweisellin A, produced by Weisella paramasenteroides Dx. Bioresource Tech. 2011;102:6730-4.
14. Routhiau VG, Raibaud P, Dubuquoy C, Moreau MC. Colonization of gnobiotic mice with human gut microflora at birth protect against E. coli heat=labile enterotoxin-mediated abrogation of oral tolerance. Pediatr Res. 2003;54:739-46.
15. Liu J, Wan R, Xu X-F, Wang XP, Yang WJ, Xia YF, et al. Effect of Lianshu preparation on lipopolysacharide-induced diarrhea in rats. World J Gastroenterol. 2009;15:2009-15.
16. Baker DG. Natural pathogens of laboratory mice, rats, and rabbits and their effects on research. Clinical Microbiology Review. 1998;11:231-66.
17. Walker WA. Mechanism of action of probiotics. Clin Infect Dis. 2008;46:S87-91.
18. Haq JA, Li HC, Rahman RA. Detection of enteropathogenic Eschericia coli (EPEC) by serotyping and cell adhesion assay among children in north-eastern Peninsular Malaysia - a hospital based study. Ibrahim Med Coll J. 2008;2:40-3.
19. Lapointe TK, O'Connor PM, Buret AG. The role of epithelial malfunction in the pathogenesis of enteropathogenic Escherichia coli-induced diarrhea. Laboratory Investigation. 2009;89:964-70.
20. Hartati AW. Evaluasi aktivitas anti diare isolat Lactobacillus dari ASI [tesis]. Bogor: Institut Pertanian Bogor; 2008.
21. Arief I, Jenie BSL, AStawan M, Witarto AB. Efektifitas probiotik Lactobacillus plantarum 2 C12 dan Lactobacillus acidophilus 2B4 sebagai pencegah diare pada tikus percobaan. Media Peternakan. 2010;33:137-43.
22. Astawan M, Wresdiyati, Arief I, Suhesti E. Gambaran hematologi tikus putih (Rattus norvenicus) yang diinfeksi Eschericia coli enteropatogenik dan diberikan probiotik. Media Peternakan. 2011;34:7-13.
23. Hsu TR, Chen SJ, Wu TC, Chung RL, Tang Rb. Tumor necroting factor alpha and interleukin 10 in viral and bacterial gastroenteritis in children. J Chin Med Asoc. 2005;68:250-3.
24. Viljanen M. Effect of probiotics bacteria on symptoms on immunologic responses in infants with atopic dermatitis [dissertation]. Helsinki: Medical Faculty of The University of Helskinsi; 2005.
25. Lomax AR, Calder PC. Probiotics, immune function, infection, and inflamation: a review of the evidence from studies conducted in human. Current Pharmaceutical Design. 2009;15:1428-518.
26. Pertiwi WA. Profil mikroflora feses dan usus tikus putih (Rattus norwegicus) dengan konsumsi daging yang difermentasi oleh Lactobacillus plantarum [tesis]. Bogor: Insitut Pertanian Bogor; 2008.
27. Kusmiati M. Aktivitas bakteriosin dari bakteri Leuconostoc mesenteroides Pbac1 pada berbagai media. Makara Kesehatan. 2002;6:1-7.
28. Heriyenni. Kajian peranan dadih susu kerbau serta campuran dadih dan virgin coconut oil terhadap performance mencit uji [tesis]. Padang: Universitas Andalas; 2007.
29. Caicedo Ra, Schanler RI, Li N, Neu J. The developing intestinal ecosystem: implications for the neonate. Pediatr Res 2005;58:625-8.
30. Guarner F, Garisch J, Eliakim R. World Gastroenterology Organisation practice guidelines: probiotics and prebiotics. Arab Journal of Gastroenterology. 2009;10:33-42.
31. Adolfson O, Meydani SN, Russel RM. Yogurt and gut function. J Clin Nutrition. 2004;80:245-56.
32. Sujaya. Isolasi dan karakterisasi bakteri asam laktat dari susu kuda SUmbawa. J Veterinal. 2008;9:52-9.
33. Melia S, Juliyarsi I. Potensi dadih susu sapi mutan Lactococcus lactis dengan kandungan bakteriosin terhadap bakteri patogen [tesis]. Padang: Universitas Andalas;2007.
34. Surono. Probiotik susu fermentasi dan kesehatan. Jakarta: Yayasan Pengusaha Makanan dan Minuman Seluruh Indonesia (YAPMMI); 2004.